Regulatory

Part:BBa_M41005:Design

Designed by: Hao Xing   Group: MIT BE 20.20-S13   (2013-05-12)


pAlkB promoter in Pseudomonas putida


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

pAlkB is regulated by different carbon sources. When alkane is the only major source for energy in the cell, pAlkB will express the operon.


Source

Van Beilen, JB, et al. "Analysis of Pseudomonas Putida Alkane-Degradation Gene Clusters and Flanking Insertion Sequences: Evolution and Regulation of the Alk Genes." Microbiology 147.6 (2001): 1621-30. Canosa, I., et al. "A Positive Feedback Mechanism Controls Expression of AlkS, the Transcriptional Regulator of the Pseudomonas Oleovorans Alkane Degradation Pathway." Molecular microbiology 35.4 (2000): 791-9.


References